Figure 6. NMuMG cells transduced with Tcf7l2 and Antxr1 show enhanced response to an exogenous WNT signal.

A) NMuMG cells stably transduced with the CIS genes were grown in control (Control CM) or WNT3A (WNT3A CM) conditioned media for 48 hr. The level of Axin2 was then examined by RT-qPCR. All values were normalized to GAPDH. Data are the means of three independent experiments with the SE values indicated by error bars. **p≤0.01, as determined by Student's t-test. B) Tcf7l2 and Antxr1-expressing cells were incubated with increasing dose of WNT3A CM for 48 hr (1, 10, 100, 1000 · l in 10 ml media) and Axin2 RNA levels were examined by RT-PCR. GAPDH was used as internal control.