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. 2011 Nov 7;6(11):e27482. doi: 10.1371/journal.pone.0027482

Figure 2. Engraftment analysis and in vitro bone resorption.

Figure 2

A) Flow cytometry analysis of peripheral blood samples stained with an antibody against CD45.2 to quantify the level of engraftment. Flow cytometry was conducted in samples from all mice (see Methods section) and at the time points indicated. B-D) Splenocytes were isolated and cultured on bovine cortical bone in the presence of RANKL and M-CSF. At day 10 bone resorption was measured by CTX-I (B) and calcium release (C) release and osteoclast numbers measured by TRACP activity in the supernatants (D). Osteoclast cultures are representative of two individual experiments with 6 replicates of each condition.