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. 2011 Nov 7;6(11):e27501. doi: 10.1371/journal.pone.0027501

Figure 2. Effect of small-dose OVA feeding on the occurrence of CD4+ TGFβRII+ T cells.

Figure 2

Mononuclear cells were isolated from PPs, MLNs, iLP, and spleens of naïve C57BL/6 mice (0) and at time points during ad libitum OVA feeding (1 mg/ml). Cells were then stained with FITC-conjugated anti-hTGFβRII, APC-labeled anti-CD4, and biotin-tagged anti-CD3 mAbs followed by PerCP-Cy™5.5-conjugated streptavidin. Samples were subjected to flow cytometry analysis by FACSCalibur®. A. Plots are representative of naïve tissues and tissues analyzed 7 days after beginning OVA feeding. B, Time course of CD4+ TGFβRII+ T cells frequency in PPs, MLNs, iLP and spleens 3, 5 and 7 days after 30 mg of OVA feeding, n = 10 mice per time point, **p<0.01 when compared to sham-tolerized control group.