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. 2011 Nov 7;6(11):e27501. doi: 10.1371/journal.pone.0027501

Figure 4. Regulatory cytokine production by CD4+ T cells of naïve and tolerized C57BL/6 mice before and after single challenge with OVA plus CT.

Figure 4

Mononuclear cells were isolated from PPs and MLNs of naive C57BL/6 mice and from mice 7 days after 30 mg of OVA feeding (0) and 8 h after single oral challenge with 1 mg of OVA plus 10 µg of CT (OVA plus CT). Cells were then stained with FITC-conjugated anti-hTGFβRII, APC-labeled anti-CD4, and biotin-tagged anti-CD3 mAbs followed by PerCP-Cy™5.5-conjugated streptavidin. Cells were stained with APC-labeled anti-CD4, PE-labeled anti-IL-10 or anti-hTGF-β1, and biotin-tagged anti-CD3 mAbs followed by PerCP-Cy™5.5-conjugated streptavidin. Samples were subjected to flow cytometry analysis by FACSCalibur®. n = 4 mice per time point, *p<0.05.