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. Author manuscript; available in PMC: 2012 Oct 10.
Published in final edited form as: Virology. 2011 Aug 19;419(1):1–9. doi: 10.1016/j.virol.2011.07.018

Fig. 2. Dominant-negative Eps15 does not inhibit Marburg GP mediated viral entry.

Fig. 2

HOS cells were plated on coverslips and transfected with mRFP-DIII (dominant-negative, DN) or D3Δ2 (control) Eps15 plasmids. 24 h post-transfection, EbGP, MARVGP pseudotyped viruses or WT-HIV was added to the cells for 4 h. 48 h post-infection, the cells were fixed and the DNA was stained with Hoechst. Several panels of images were collected from each coverslip. The number of transfected only and transfected and infected cells was counted in each panel and the percentage of transfected and infected cells was determined. Graph represents % decline in viral infectivity in DN Eps15 transfected cells normalized to control Eps15 transfected cells. Error bars represent SEM for three independent experiments. * p Value for % decline in EbGP infectivity compared to MARVGP and HIV is < 0.01.