Skip to main content
. 1999 Mar;119(3):873–884. doi: 10.1104/pp.119.3.873

Figure 3.

Figure 3

A, Northern-blot hybridization using the full-length T106 probe of 10 μg of total RNA of vegetative mt+ (V+) and mt− (V−) cells, mt+ (G+) and mt− (G−) gametes, and zygotes at 0, 10, and 30 min and 1, 2, 4, and 6 h. B, Location of the ankyrin repeats on clone T106 and of the deleted sequence on clone T91. The region selected as specific probes for T91 or T106 and resultant fragments degraded by RNase A are indicated as solid lines. C, Difference in signal patterns in the RNase protection assay applied to total RNA of the zygote at 4 h with either specific probe. D, Survey of the temporal expression patterns of Zys3 mRNA by the RNase protection assay using the partial sequence specific for cDNA clone T91. HincII-digested φX174 DNA was used as a molecular marker.