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Temporal expression pattern of Zys3 proteins. Total protein samples of C. reinhardtii vegetative mt+ (V+) and mt− (V−) cells, mt+ (G+) and mt− (G−) gametes, and zygotes at 0, 10, and 30 min and 1, 2, 4, 6, and 22 h were fractionated, blotted, and probed with α-Zyspept3. Strong signals were detected in the vicinity of 40 kD at 2, 4, 6, and 22 h. A slightly heavier minor band that appeared at the same time may be the Zys3 precursor containing the signal peptide.
