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Variation of volume V for two protoplasts, after an osmotic change from isotonic OP1 to hypoosmotic OP2 or hyperosmotic OP3 medium. Protoplasts were obtained from 3-d-old rape roots. V0, Initial volume under isotonic conditions. OP1 = 400 mosmol kg−1; OP2 = 200 mosmol kg−1; and OP3 = 600 mosmol kg−1.
