Table I.
Maximal increase in surface area during measurements for hypocotyl and root protoplasts submitted to hyposmotic stress
| Plant | (Smax −
SI)/SI
|
|
|---|---|---|
| Hypocotyl (5 d) | Root (3–5 d) | |
| Rape | 0.14 ± 0.06 (5) | 0.34 ± 0.06 (13) |
| Flax | 0.15 ± 0.16 (9) | 0.34 ± 0.03 (4) |
The 60- to 100-μm-diameter spherical protoplasts were transferred from 450 to 250 mosmol kg−1 solutions. Smax was measured after 2 min or immediately before bursting. Membrane disruption was observed for all but one hypocotyl protoplast. (Smax − SI)/SI (mean ± sd): relative change of the membrane surface, referred to initial value SI of the protoplast. The number of experiments is given in parentheses.