Figure 5.

NRP1 knockdown abrogates apoptosis induced by VEGF189 under serum starvation conditions. Annexin V/PI staining was performed on adherent cells harvested after 72 h incubation in 0.5% FBS-containing medium. Histograms of means (±SE M) (left) and representative flow cytometry profiles (right) stand for transfected with shNRP-1 vs. irrelevant shCtl clones overexpressing VEGF189 (A), n = 3; VEGF 165 (B), n = 4; and control cV (C), n = 3. Two clones were tested for each shRNA transfection (see Table 2B) giving similar profiles; shown results stand for cVshNRP1–19, V189shNRP1–10 and V165shNRP1–5. *p ≤ 0.05 vs. corresponding shCtl clone.