Figure 8.

Analysis of the expression and activity of uPA in the different clones. (A) NRP1 knockdown decreases uPA expression in VEGF189 overexpressing clones. Cells were maintained in 10% FBS containing medium. Transcripts were analyzed by qRT-PCR. Results are normalized to their corresponding shCtl clones. Values represent means ± SE M of three independent experiments. *p < 0.05 vs. corresponding shCtl clone; (B) NRP1 knockdown results in decreased uPA activity in VEGF189 overexpressing clones. Conditioned medium from cells cultured for 24 h in serum-free DMEM was analyzed using zymography, as described in Materials and Methods. The figure shows a representative from two independent experiments.