Table 2.
(A) | Clones number | VEGF165 | VEGF189 | NRP1 |
cV | 1 | 1 | 1 | |
Clones | V189 | 1.5 | 30.5# | 1 |
V165 | 48# | 1.4 | 1.4 | |
(B) | Clones number | VEGF165 | VEGF189 | NRP1 |
shCtl | 1 | 1 | 1 | |
cV clones | shNRP1–9 | 0.9 | 0.8 | 0.6* |
shNRP1–19 | 0.5 | 0.5 | 0.4* | |
shCtl | 1 | 1 | 1 | |
V189 clones | shNRP1–10 | 0.4* | 9.7* | 0.6* |
shNRP1–11 | 0.48* | 5.8* | 0.57* | |
shCtl | 1 | 1 | 1 | |
V165 clones | shNRP1–3 | 1 | 0.5 | 0.5* |
shNRP1–5 | 0.7 | 0.6 | 0.4* |
(A) VEGF overexpressing clones. Expression of VEGFmRNA was assessed using real-time PCR V189 clones (V189–13), V165 (V165–42) clones, and cV (cV-14) clones (for VEGF189 and VEGF165 overexpressing, or control clones, respectively), were initially described in reference 10; these clones were maintained in 10% SVF condition Data obtained using real-time PCR were expressed as mean values, as compared with cV clones set to 1. (B) ShNRP1 and shCtl transfected clones. Data, obtained in 10% FBS containing medium, were expressed as mean values of 4 independent experiments performed in duplicate, as compared with corresponding shCtl clone set to 1. Statistical significance (p < 0.05):
VEGF overexpressing vs. cV clone;
shNRP1 clone vs. shCtl clone.