Table 4.

Comparison of VEGF189 mRNA levels in different conditions

	Clones number	10% FBS	0.5% FBS	Hypoxia
cV	shCtl	1	1	1
clones	shNRP1–19	0.5	0.8	0.58
V189	shCtl	30.5	36	33.4
clones	shNRP1–10	297*	263*	136*
V165	shCtl	1.4	0.5	3.6
clones	shNRP1–3	0.7	0.6	1.1

Sh-transfected V189, V165 or cV clones, as described in Materials and Methods, were maintained in medium containing 10% FBS, 0.5% FBS (72 h serum starvation conditions), or under hypoxia (for 24 h in 1% O₂, 4.5 g/L glucose and 10% FBS). Analysis of transcripts was performed using qRT-PCR. Results are expressed as fold induction, as compared with cV shCtl clone set to 1 in corresponding condition. Note that NRP1 knockdown resulted in 10-fold increased VEGF189 expression (297 vs. 30) in 10% FBS (*p < 0.05).