Table 4.
Clones number | 10% FBS | 0.5% FBS | Hypoxia | |
cV | shCtl | 1 | 1 | 1 |
clones | shNRP1–19 | 0.5 | 0.8 | 0.58 |
V189 | shCtl | 30.5 | 36 | 33.4 |
clones | shNRP1–10 | 297* | 263* | 136* |
V165 | shCtl | 1.4 | 0.5 | 3.6 |
clones | shNRP1–3 | 0.7 | 0.6 | 1.1 |
Sh-transfected V189, V165 or cV clones, as described in Materials and Methods, were maintained in medium containing 10% FBS, 0.5% FBS (72 h serum starvation conditions), or under hypoxia (for 24 h in 1% O2, 4.5 g/L glucose and 10% FBS). Analysis of transcripts was performed using qRT-PCR. Results are expressed as fold induction, as compared with cV shCtl clone set to 1 in corresponding condition. Note that NRP1 knockdown resulted in 10-fold increased VEGF189 expression (297 vs. 30) in 10% FBS (*p < 0.05).