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. 2011 Jul 1;5(4):344–350. doi: 10.4161/cam.5.4.16717

Figure 4.

Figure 4

A complex interrelationship exists between Tuba and N-WASP at the PAP and requires the polyproline region of N-WASP. (A) N-WASP localizes to the cell-cell junction in control and Tuba KD cells. (B) Tuba is observed to localize to the cell-cell junction in control cells and cells reconstituted with an N-WASP mutant in which the VCA domain has been deleted. Tuba does not localize to the cell-cell membrane in N-WASP KD cells or cells reconstituted with an N-WASP mutant in which the polyproline region has been deleted. (C) N-WASP localization in two cell cysts. 93.6% of control cells show accumulation of N-WASP at the cell-cell junction in two cell cysts compared to 70.9 ± 5.2% of Tuba KD cysts (p < 0.0001; Student's t-test), 6.4 ± 3.3% of N-WASP KD cysts (p < 0.0001; Student's t-test), 98.8 ± 1.2% of ΔVCA N-WASP cysts (p = 0.0071; Student's t-test) and 80.4 ± 8.8% of ΔPP N-WASP cysts (p = 0.0167; Student's t-test). (D) Tuba localization in two cell cysts. 89.3% of control cells show accumulation of Tuba at the cell-cell junction in two cell cysts compared to 21.9 ± 3.2% of N-WASP KD cysts (p < 0.0001; Student's t-test), 99.3 ± 0.7% of ΔVCA N-WASP cysts (p = 0.0149; Student's t-test) and 37.7 ± 2.6% of DPP N-WASP cysts (p < 0.0001; Student's t-test). (Data are means ± SEM of data pooled from at least 3 individual experiments). Scale bars = 10 µm.