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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Oct 23;18(11):1244–1249. doi: 10.1038/nsmb.2135

Figure 2.

Figure 2

Direct visualization of erbB1 dimerization is captured by two-color SPT. (a) Sample time series showing dimer formation (white) between QD585-EGF-erbB1 (green) and QD655-EGF-erbB1 (magenta). (b) Cartoon of tracking condition (top), 3D trajectories (middle), and distance between receptors (bottom) as a function of time are shown for the indicated receptors in (a). (c) Sample time series for QD655-EGF-erbB1 and QD585-VHH-erbB1 shows interactions for a 1:2 EGF:erbB1 dimer. (d) Cartoon of tracking condition (top), 3D trajectories (middle), and distance between receptors (bottom) as a function of time are shown for the receptors in (c). Scale bar, 0.5 μm for (a) and (c). (e-h) Ensemble correlated motion plots summarize all two-color data for EGF, EGF+PD, and VHH conditions. A decrease in uncorrelated jump distance (blue) at short separations indicates that receptors are moving together. A concurrent drop in jump magnitude (red) demonstrates decreased diffusion.