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. 2011 Nov 4;44(3):361–372. doi: 10.1016/j.molcel.2011.08.032

Figure 5.

Figure 5

Zfp57 Removal Leads to a Loss of DNA Methylation and ZFP57/KAP1 Interaction with NP95 and DNMTs

(A) Bisulfite sequencing analysis of DNA extracted from Zfp57 knockout and WT ES cells. ICR, but not IAP, DNA methylation is lost in Zfp57 knockout ES cells. Differences between Zfp57−/− and WT ES cells are statistically significant (p < 0.005).

(B) Total protein extracts (input) or immunoprecipitates recovered with either naked (IP: beads) or antibody-coated beads with indicated antibodies (IP:KAP1 and IP:Flag) from control (Mock, left) or Flag-NP95-expressing ES cells were analyzed by western blot with antibodies, indicated on the right.

(C) Same experiment in WT ES cells, using control, KAP1, and HA-specific beads for the IP and KAP1, HA, DNMT1, DNMT3A, or DNMT3B-specific antibodies for the western blot.