. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 18;879(30):3465–3474. doi: 10.1016/j.jchromb.2011.09.026

TABLE 3.

Limits of detection (LOD), limits of quantification (LOQ), upper limits of quantification (ULOQ) and calibration curve results (N=11) of nicotine and metabolites in rat brain.

Analyte	LOD (ng/mg)	LOQ (ng/mg)	ULOQ (ng/mg)	R²
3-HC	0.010	0.025	7.5	0.997
AB	0.010	0.100	7.5	0.995
AT	0.010	0.025	7.5	0.997
CNO	0.010	0.025	7.5	0.994
COT	0.010	0.025	7.5	0.999
COTGLUC	0.010	0.100	7.5	0.990
NCOT	0.010	0.025	7.5	0.999
NIC	0.010	0.100	7.5	0.999
NIC GLUC	0.010	0.025	7.5	0.998
NNIC	0.010	0.025	7.5	0.998
NNO	0.010	0.025	7.5	0.994

Signal to noise ratio was used to determine LOD (≥3 and ≤10) and LOQ (>10).

Results come from 4 batches analyzed among 4 different days.