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Brassinosteroid-biosynthetic step found to be defective in dwf1 plants. The aerial parts of 5-week-old wild-type and dwf1 plants were subjected to analysis of endogenous brassinosteroid levels using GC-SIM. Accumulation of 24-methylenecholesterol with a simultaneous reduction in the campesterol level suggests that a specific step, C-24 reduction, was blocked in dwf1 mutants. Campesterol is known to serve as substrate for brassinolide biosynthesis.
