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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Insect Biochem Mol Biol. 2011 Sep 29;41(12):946–955. doi: 10.1016/j.ibmb.2011.09.004

Figure 3.

Figure 3

Metabolic labeling experiments using 14C-Glucose and 14C-Leucine show that de novo lipid biosynthesis is inhibited in blood fed ACC and FAS1 deficient mosquitoes. A) Accumulation of 14C-TAG (triacylglycerol) and 14C-PL (phospholipid) in ACC and Fluc dsRNA injected mosquitoes at 48 hr PBM using blood meals containing 14C-Glucose or 14C-Leucine. Data are shown as Mean ± SEM from three independent biological replicas using whole body lipid extracts. B) Accumulation of 14C-TAG and 14C-PL in FAS1 and Fluc dsRNA injected mosquitoes at 48 hr PBM using blood meals containing 14C-Leucine. Data are shown as Mean ± SEM from three independent biological replicas using whole body lipid extracts. Asterisks indicate a significant difference between 14C-lipid levels in FAS1 and Fluc dsRNA injected mosquitoes (*P<0.05; ***P < 0.001).