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Oocytes in ACC deficient mosquitoes lack an intact eggshell layer. Oocytes were collected from Fluc and ACC dsRNA injected mosquitoes, and either fixed with glutaraldehyde and stained with toluidine blue to visualize eggshell structure by high magnification light microscopy, or stained in water with neutral red or rhodamine B. Tubercle structures (Tub) and vitellogenin granules (Vg) are labeled. Bar scale is equal to 20 μm.
