Table 4. CT values for mRNA transcripts in ovarian tissue (mean CT value).
| Tissue sample |
γ-SYN | ERαΔ3 | ERα | β-Actin |
|---|---|---|---|---|
| B1 | 28.3 | 37.2 | 31.0 | 19.9 |
| OV1 | 30.3 | 45.4 | 38.3 | 24.9 |
| OV2 | 29.6 | 34.3 | 28.9 | 22.7 |
| OV3 | 29.8 | 37.3 | 33.5 | 23.8 |
|
| ||||
| B2 | 29.3 | 42.1 | 35.1 | 21.6 |
| OV4 | 31.7 | 43.4 | 35.8 | 23.7 |
| OV5 | 32.3 | 35.0 | 28.3 | 21.8 |
| OV6 | 29.2 | 36.2 | 30.2 | 20.7 |
| OV7 | 25.0 | 41.8 | 35.1 | 21.7 |
|
| ||||
| B3 | 27.3 | 38.0 | 29.9 | 21.4 |
| OV8 | 22.9 | 35.9 | 29.7 | 20.5 |
| OV9 | 28.4 | 32.3 | 28.1 | 20.5 |
| OV10 | 25.4 | 36.0 | 29.6 | 20.7 |
| OV11 | 23.5 | 32.6 | 27.0 | 20.3 |
|
| ||||
| B2 | 31.8 | 43.9 | 36.5 | 23.3 |
| B1 | 30.5 | 38.9 | 32.3 | 20.3 |
| B3 | 29.8 | 38.1 | 32.9 | 22.9 |
| B4 | 30.1 | 36.1 | 30.2 | 23.1 |
| B5 | 28.5 | 39.6 | 33.6 | 22.5 |
| B6 | 29.0 | 37.5 | 33.0 | 22.5 |
| B7 | 29.8 | 38.5 | 32.7 | 23.3 |
|
| ||||
| B2 | 28.7 | 39.5 | 32.7 | 21.0 |
| B8 | 29.2 | 41.2 | 35.1 | 24.0 |
| B9 | 27.4 | 40.5 | 34.3 | 25.6 |
| B10 | 26.6 | 34.9 | 28.6 | 21.2 |
| B11 | 27.1 | 36.8 | 30.6 | 20.4 |
| B12 | 26.5 | 36.4 | 30.9 | 20.3 |
| B13 | 35.4 | 33.7 | 31.3 | 27.3 |
This table presents the raw data required for the relative gene expression analysis shown in Table 3. The mean CT values of each gene investigated and the mean CT value of β-ACTIN (in brackets), is given for each patient. Gene expression analysis is performed by comparing β-ACTIN values with those of the gene of interest, relative to one ‘control‘ patient known as the calibrator in order to determine relative inter-patient differences. In this case benign tissues (patients B1, B2 or B3) were chosen as the calibrator control and consequently assigned a gene expression value of 1 in subsequent analyses (see Table 3A).