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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Placenta. 2011 Sep 1;32(11):852–858. doi: 10.1016/j.placenta.2011.08.003

Figure 1. Immunofluorescence and histopathology analyses of placental lipid loading and apoptosis in placentas from normal and assisted fertilization pregnancies.

Figure 1

1A: Representative immunofluorescent images of mouse placentas stained with Nile Red at 10× magnification oriented with the chorionic plate to the right in all slides (scale bar = 250 μm) (i) Normal fertilization, (ii) IVF, (iii) ICSI. 1B: Semi-quantification of fluorescence using fluorescence:tissue area pixel ratio. Bars are means ± SEM. 1C: Semi-quantification of apoptosis measured by TUNEL staining:tissue area pixel ratio analyzed in Image J. 1D: Representative micrographs of TUNEL staining in the mouse placenta at 4× magnification (i) Normal fertilization, (ii) IVF, (iii) ICSI. Scale bar = 1 mm. Bars are means ± SEM. * = P < 0.05, ** = P < 0.01 vs. normal fertilization, t-test. Abbreviations S = syncytium, L = labyrinth zone. NS = not significant.