Table 1.
Activities of antioxidant enzymes in the maternal-placental-fetal unit in ART.
| TISSUE | CAT μmol | GPx nmol | GR nmol | GST nmol | SOD U | TrxR nmol | XO fmol | |
|---|---|---|---|---|---|---|---|---|
| Maternal Liver | Normal | 6.6 ± 3.5 | 325 ± 65 | 2.1 ± 0.3 | 117 ± 16 | 3.1 ± 1.4 | 4.7 ± 2.1 | 22 ± 1.2 |
| IVF | 5.1 ± 0.6 | 385 ± 96 | 1.6 ± 0.3 | 198 ± 29 * | 2.9 ± 1.0 | 4.7 ± 2.4 | 21 ± 2.6 | |
| ICSI | 9.1 ± 2.9 | 482 ± 108 | 2.2 ± 0.2 | 92 ± 14 | 3.4 ± 1.0 | 8.8 ± 4.3 | 17 ± 1.1 | |
| Placenta | Normal | 1.2 ± 0.1 | 251 ± 10 | 1.1 ± 0.1 | 19 ± 2.8 | 3.2 ± 0.6 | 17 ± 0.6 | 50 ± 4.0 |
| IVF | 1.4 ± 0.1 | 255 ± 9 | 0.9 ± 0.08 | 17 ± 1.5 | 1.5 ± 0.1 *** | 11 ± 0.4 *** | 23 ± 1.3 *** | |
| ICSI | 0.9 ± 0.03 * | 203 ± 5 *** | 0.6 ± 0.06 ** | 11 ± 0.8 * | 1.7 ± 0.2 * | 12 ± 0.9 *** | 27 ±2.4 *** | |
| Fetal Liver | Normal | 1.2 ± 0.2 | 280 ± 25 | 1.6 ± 0.25 | 39 ± 4.6 | 1.0 ± 0.2 | 4.6 ± 0.5 | 3.3 ± 0.4 |
| IVF | 1.7 ± 0.2 | 245 ± 20 | 0.9 ± 0.13 * | 31 ± 2.6 | 1.0 ± 0.1 | 5.2 ± 0.5 | 3.4 ± 0.4 | |
| ICSI | 1.4 ± 0.1 | 259 ± 21 | 0.7 ± .12 ** | 29 ± 4.1 * | 0.6 ± 0.1 * | 6.2 ± 0.9 | 3.0 ± 0.3 |
aAll enzyme activities are normalized to min−1mg protein−1 and expressed as mean ± SEM, with n= 3 for materna livers from each group and n = 20, n = 31 and n = 21 matched samples for placentas and fetal livers from normal fertilization, IVF and ICSI pregnancies, respectively. Abbreviations: μmol; micromoles, nmol; nanomoles, fmol; femtomoles, U = Units (amount of enzyme required to inhibit 50% of dismutation of superoxide). Statistical significance:
*
P < 0.05
**
P < 0.01
***
P < 0.001 vs. normal tissues, and significant changes are bolded.