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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Biophys Chem. 2011 Sep 8;160(1):12–19. doi: 10.1016/j.bpc.2011.08.006

Figure 4.

Figure 4

Inhibition of zinc-promoted oligomerization at pH 7.4. Each distribution represents at least 100 small FAβB species from multiple samples, interrogated one at a time. The total percentage of monomer and each oligomer observed (up to n = 5) is plotted in order to facilitate comparison between different sample environments; each sample additionally contains ~1–5% larger oligomers, not shown. A) As we have reported previously [50], the presence of 4 equivalents of Zn2+ (black bars) significantly shifts the distribution of FAβB monomers and oligomers toward larger species, as compared to nominally metal-free samples at pH 7.4 (white bars). B) The addition of ten molar equivalents of any inhibitor to samples containing 4 equivalents of Zn2+ (pH 7.4) significantly shifts the distribution of FAβB species toward monomers, as compared to inhibitor-free samples (Panel A). Aβ16–22m and KLVFF-K6 are most successful in producing monomers of FAβB. Inset: Aβ16–22m and KLVFF-K6 were tested at concentrations equimolar with FAβB. Each successfully shifts the distribution of FAβB species toward monomers at this reduced concentration; as was observed for 10 equivalents of inhibitor, the two distributions are statistically indistinguishable.