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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2011 Sep 6;82(12):1940–1949. doi: 10.1016/j.bcp.2011.08.026

Figure 1.

Figure 1

The pGL4.3xERE reporter construct is estrogen responsive. (A) Three tandem EREs were inserted upstream of the luc2P gene in the pGL4.32 luciferase reporter construct. HEK293 cells were batch transfected with the pGL4.3xERE reporter construct, a β-galactosidase construct, and full length ERα (B) or ERβ (C). After allowing 24 hr for protein expression, cells were treated in triplicate with vehicle (DMSO) or 1 nM E2 and vehicle or 100 nM ICI 182,780 (0.15 % final DMSO concentration) for an additional 24 hr. Raw luciferase units (RLUs) were normalized to β-galactosidase to normalize for transfection efficiency. Error bars represent standard deviations.