Skip to main content
. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Biochem Pharmacol. 2011 Sep 6;82(12):1940–1949. doi: 10.1016/j.bcp.2011.08.026

Figure 2.

Figure 2

ER subtype selective ligands selectively induce luciferase in Hs578T-ERαLuc and Hs578T-ERβLuc cells. Hs578T-ERαLuc (A) and Hs578T-ERβLuc (B) cells were seeded in triplicate on 96 well plates in the presence of 50 ng/mL Dox to induce ER expression. After 24 hr, cells were treated with vehicle (DMSO), 1 nM E2, 10 nM DPN, or 10 nM PPT in the presence or absence of 100 nM ICI 182,780 (0.15% final DMSO concentration). Cells were lysed 24 hr after ligand treatment and raw luciferase units were counted. Error bars represent standard deviations. * p values < 0.05