Figure 4.
Effect of chronic food restriction on phosphorylation of ERK 1/2 and Akt under basal conditions in ad libitum (AL) fed and food-restricted (FR) rats. Lysates were immunoblotted with anti-phospho p44/42 MAPK, anti-p44/42 MAPK, or anti-phospho-Ser473-Akt antibodies. Following densitometry, intensities of bands corresponding to phospho-proteins for each subject were divided by the intensities of the corresponding total protein (ERK or Akt) bands to correct for small differences in protein loading. Results (mean ± S.E.M.) are expressed in comparison to the normalized control, which was defined as the ad libitum fed group injected with vehicle. Graphed results are displayed with representative immunoblots. (nAL = 6; nFR = 6), *p ≤.05