Figure 4.
A high resolution DDD reconstruction from a 72,316 particle dataset is shown in (A), low pass filtered to 4.5Å. In (B), the Fourier Shell Correlation, derived from reconstructed even-odd particle data sets, gives the FSC0.5 as 6.1Å. For comparison, we show an asymmetric subunit from the DDD map in (C) and previously published asymmetric subunits from a 4Å map acquired using film in (D) (Ludtke et al., 2008) and a 5.4Å map from a CCD camera in (E) (Stagg et al., 2008).