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. 2011 Oct 6;44(5):213–221. doi: 10.1267/ahc.11009

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© 2011 The Japan Society of Histochemistry andCytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — The transduction efficiency of lentiviral vector in the bone marrow-derived mesenchymal stem cells. (A) Merged image of the GFP-positive cells and DAPI staining of the bone marrow-derived mesenchymal stem cells. (B) Superimposition of images of (A) and differential interference contrast optic image. Arrowheads indicate cells that are GFP-negative. (C) The graph shows the percentage of the total number of GFP-positive human bone marrow-derived mesenchymal stem cells during the culture. The percentage of GFP-positive cells gradually decreased between days 7 and 28. There was statistically significant difference among the three group means as determined by one-way ANOVA test (P<0.05). Results are mean±SEM. Bar=100 µm.