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. 2011 Oct 6;44(5):213–221. doi: 10.1267/ahc.11009

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© 2011 The Japan Society of Histochemistry andCytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Bar graphs showing the percentage of differentiated and integrated GFP-positive human bone marrow-derived mesenchymal stem cells. GFP-positive human bone marrow-derived mesenchymal stem cells intraocularly injected in the 1PN/5PN/10PN mouse retinas. One week after transplantation, transplanted cells showed morphological and phenotypic differentiation and integration into retina. (A) Quantification of morphologically differentiated GFP-positive human bone marrow derived-mesenchymal stem cells in vitreous and retina. There was statistically significant difference among the three group means as determined by one-way ANOVA test (P<0.05). (B) Quantification of GFP-positive human bone marrow derived-mesenchymal stem cells co-expressing MAP2ab or GFAP in vitreous and retina one week after intraocular transplantation. The asterisks indicate no statistically significant difference between GFP-positive cells co-expressing MAP2ab and GFP-positive cells co-expressing GFAP at the same postnatal age. (C) Quantification of integrated GFP-positive human bone marrow derived-mesenchymal stem cells into retina. There was statistically significant difference among the three groups as determined by one-way ANOVA test (P<0.05). Results are mean±SEM. Percentage of differentiated or integrated cells calculated as the number of differentiated or integrated cells that expressed GFP, divided by total number of surviving GFP-positive transplanted cells ×100.