Phenotype of MV clones. A, Phase-contrast images of MVEC-4 and MVIC-7. B and C, Immunofluorescence staining of MVEC-4 and MVIC-7 for VE-cadherin (B) and for α-SMA (C). D, Cells immunostained simultaneously for CD31 and α-SMA and analyzed by flow cytometry. E, Cells stained for CD90 only. F, Cells stained with isotype-matched phycoerythrin- and fluorescein isothiocyanate–conjugated control antibodies. G, Western blot for CD31, VE-cadherin, and tubulin in MVEC-4, MVEC-5, and MVIC-7.