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Osteogenic endothelium in vivo. A, Ovine mitral valve leaflets from sham-operated animals and animals in which mechanical stretch was applied to the mitral valve for 2 months were analyzed for CD31 and osteocalcin (n=2) by immunohistochemistry. B, “Stretched” ovine mitral valve leaflets were double-labeled with anti-osteocalcin and anti-CD31 (top images), anti–α-SMA and anti-CD31 (middle images), or with anti–α-SMA and anti-osteocalcin (bottom images). Magnification=×400.
