Table.
Oligonucleotide Primer Sequences Used for PCR
| Gene | Accession No. | Oligonucleotides | Product Size (bp) |
|---|---|---|---|
| Osteopontin (human) | NM_001040060.1 | 5’-CCAAGTAAGTCCAACGAAAG-’3 | 347 |
| 5’-GGTGATGTCCTCGTCTGTA-’3 | |||
| Osteopontin (sheep) | AF152416.1 | 5’-CTGATTTTCCCACTGACATT-’3 | 354 |
| 5’-CTATGGAATTCTTGGCTGAG-’3 | |||
| Osteonectin (human and sheep) | NM_001166202.1 | 5’-CGACTCTTCCTGCCACTTCT-’3 | 63 |
| 5’-TTGTGGCCCTTCTTGGT-’3 | |||
| Osteocalcin (human) | X51699 | 5’-ATGAGAGCCCTCACACTCCTC-’3 | 293 |
| 3’- GCCGTAGAAGCGCCGATAG-’3 | |||
| Osteocalcin (sheep) | NM_001040009.1 | 5’-AGCTCATCACAGTCAGGGTTG-’3 | 121 |
| 3’- AGCGAGGTGGTGAAGAGA-’3 | |||
| Peroxisome proliferator-activated receptorγ 2 (human) | NM_015869.4 | 5’-GCTGTTATGGGTGAAACTCT-’3 | 116 |
| 5’-TCGCAGGCTCTTTAGAAACTC-’3 | |||
| Peroxisome proliferator-activated receptorγ 2 (sheep) | AY137204.1 | 5’-ATGTCTCATAATGCCATCAGGTT-’3 | 224 |
| 5’-GATAACAAACGGTGATTTGTCTGTC-’3 | |||
| Collagen type 2 (human) | NM_001844 | 5’-CTCCTGGAGCATCTGGAGAC-’3 | 142 |
| 5’-ACCACGATCACCCTTGACTC –’3 | |||
| Collagen type 2 (sheep) | FJ378650 | 5’-CGTCACCTACCACTGCAAGA-’3 | 134 |
| 5’-CGGTGTATGTGAACCTGCTG-’3 | |||
| SRY-box containing gene 9 (human and sheep) | NM_000346.3 | 5’-TCTGAACGAGAGCGAGAAGC-’3 | 81 |
| 5’-GTAATCCGGGTGGTCCTTCT-’3 | |||
| PECAM-1 (human) | NM_000442 | 5’-CAGTCAGAGTCATTCTTGCC-’3 | 176 |
| 5’-GTTGTTGGAGTTCAGAAGTGG-’3 | |||
| PECAM-1 (sheep) | GQ268014.1 | 5’-GTTCAGCGAAGTTCTGCGAG –’3 | 228 |
| 5’-CTTGCTGGCTGTGGTCTTGT-’3 | |||
| Glyceraldehyde-3-phosphate Dehydrogenase (human and sheep) | NM_002046 | 5’-TGCACCACCAACTGCTTAG-’3 | 172 |
| 5’-GATGCAGGGATGATGTTC-’3 |