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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Cancer Res. 2011 Feb 9;71(7):2654–2663. doi: 10.1158/0008-5472.CAN-10-2889

Figure 6. Suppression of autophagy increases the cytocidal activity of MK-2206 in glioma cells.

Figure 6

(A) LN229 and T98G cells were transfected with a non-targeting RNA or a siRNA targeting eEF-2 kinase, followed by treatment with a series concentration of MK-2206 for 48 h in fully supplemented medium. (B) LN229 and T98G cells cultured in medium supplemented with 10% fetal bovine serum were treated with a series concentration of MK-2206 for 48 h in the presence or absence of 0.25 μM of NH125. (C) LN229 and T98G cells were transfected with a non-targeting siRNA or a beclin 1-targeted siRNA, followed by treatment with a series concentration of MK-2206 for 48h. (D) LN229 and T98G cells were treated with a series concentration of MK-2206 for 48 h in the presence or absence of 1 mM of 3-MA. At the end of treatment, cell viability was measured by MTT assay. Results shown were mean ± SD of quadruplicate determinations from one of three identical experiments; *p< 0.05, **p< 0.01.