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. 2011 Jan 12;3(1):82–104. doi: 10.3390/toxins3010082

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© 2011 by the authors; licensee MDPI, Basel, Switzerland

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure S1 — Disruption of the msnA gene in A. parasiticus and A. flavus by the ptr selectable marker. (A) Diagram depicting the gene disruption event via double-crossover recombination. I: linearized portion of the disruption vector; II: genomic pattern of the recipient strain; III: expected genomic pattern after recombination. (B) PCR confirmation of genomic DNA patterns of the recipient, R, and the msnA disruptant, D. The primers used were P1 and P2. The DNA size markers (in kb) are lambda DNA/Hind III fragments. The three sets are 1: A. parasiticus BN9, 2: A parasiticus RH and 3: A. flavus CA14.