Figure 2.

Amino acid sequence comparison of the TPM1 gene from six animal species. Amino acid alignment of all exons contained within the TPM1 gene for selected members of chordata. Species included are human (Homo sapiens), mouse (Mus musculus) and rat (Rattus norviegicus) representing mammalians, chicken (Gallus gallus) representing birds, western clawed frog (Xenopus tropicalis) representing amphibians and zebrafish (Danio rerio) representing fish. Shaded areas and consensus sequence indicate regions of sequence conservation while unshaded regions represent areas of sequence divergence between the aligned species. Dashed lines within alignments imply that either the particular exon is not found within that species or that the exon in question has not been identified due to minimal genomic sequence information and lack of evidence from cDNA library sequences. Zebrafish contains two copies of the TPM1 gene named TPM1 and TPMa which are the result of a whole genome duplication event. Antibody immunogens and/or epitopes for antibodies TM311, α/2a, α/1b, α/9b, α/9c and α/9d are presented as white text highlighted in red and are aligned with the region of the exon from which they were derived.