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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2011 Nov;31(11):e72–e79. doi: 10.1161/ATVBAHA.111.230938

Figure 1.

Figure 1

Derivation and characterization of hiPSC-ECs. A) [Left panel] Isotype control. [Right panel] FACS plot of data obtained using CD31+ antibody to quantitate ECs derived from hiPSCs B) to E) Immunofluorescent staining of the hiPSC-ECs for endothelial markers CD31, CD144, eNOS and vWF. F) hiPSC-ECs form a capillary-like network on Matrigel 24 hours after seeding the cells. G) hiPSC-ECs take up acetylated LDL. Scale bar: 50μm. H) in vivo formation of capillary structures in matrigel plugs after subcutaneous transplantation in SCID mice, stained using an Ab specific for human CD31.