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. Author manuscript; available in PMC: 2011 Nov 8.
Published in final edited form as: Nat Cell Biol. 2010 Jul 18;12(8):739–746. doi: 10.1038/ncb2087

Figure 1. mCherry-CtpS is dynamic and co-localizes with linear filamentous structures along the inner curvature of Caulobacter cells.

Figure 1

(a) mCherry-CtpS localization in asynchronous Caulobacter cells. mCherry-ctpS expressing cells (ZG153) were induced for two hours with xylose and imaged. A merged phase and fluorescence image is shown. Scale bar represents 2 μm. (b) mCherry-CtpS localization in synchronized Caulobacter cells. mCherry-ctpS expressing cells (ZG153) were induced for two hours with xylose, synchronized, and imaged at 10-minute intervals in the presence of xylose. Merged phase and fluorescence (top), fluorescence (middle), and cartoon depictions (bottom) are shown for two fields of representative cells at 0, 50, 80, and 110 minutes from the beginning of the timelapse. Scale bar represents 2 μm. (c) Co-localization of mCherry-CtpS and filamentous structures in Caulobacter. mCherry-ctpS expressing cells (ZG153) were fixed on EM grids and imaged first by fluorescence light microscopy and then by ECT. Shown is an ECT slice, phase-fluorescence overlay (left inset), and cartoon depiction (right inset) of the same cells. These cells correspond to cells #15 from the field shown in Figure S2. Arrows point to the ends of the filaments in the ECT image, which correspond to the positions of the mCherry-CtpS structure shown in the inset. Scale bars represent 100 nm for EM and 1 μm for the inset LM images.