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. Author manuscript; available in PMC: 2011 Nov 8.
Published in final edited form as: Nat Cell Biol. 2010 Jul 18;12(8):739–746. doi: 10.1038/ncb2087

Figure 4. CtpS regulates cell shape through an interaction with crescentin.

Figure 4

(a–d) Cell morphology of Caulobacter cells with wild-type (a and b) and depleted (c and d) CtpS levels in a background either with creS (a and c, creS+, ZG215) or without creS (b and d, ΔcreS, ZG216). (e–f) Localization of Caulobacter mCherry-CtpS (red) in creS+ (e, ZG287) and ΔcreS (f, ZG285) backgrounds. Merged fluorescent images are shown, and the cell periphery is marked with a gfp fusion (green) to the periplasmic protein encoded by CC2395. (g) ECT filaments in ΔcreS cells (ZG17). Arrows point to filaments ends. (h–i) Co-localization of Caulobacter mCherry-CtpS (red) and CreS-tc (green) in both Caulobacter (h, ZG218) and upon heterologous co-expression in E.coli (i, ZG221). White scale bars represent 2 μm, black scale bars represent 100 nm.