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. Author manuscript; available in PMC: 2011 Nov 8.
Published in final edited form as: Nat Cell Biol. 2010 Jul 18;12(8):739–746. doi: 10.1038/ncb2087

Figure 5. The E. coli CtpS homolog forms filaments both in vivo and in vitro.

Figure 5

(a) Localization of an mCherry fusion to the E. coli CtpS homolog (referred to here as EcCtpS but also known as pyrG) in E. coli. mCherry-EcCtpS expressing cells (ZG283) were induced for two hours with IPTG and imaged. Merged phase fluorescent (left) and fluorescent (right) images are shown. (b) Localization of the endogenous, untagged E.coli CtpS. Immunofluorescence microscopy was performed on wild type E. coli cells (NCM3722) probed with an α CtpS antibody. Merged phase and fluorescent (left) and fluorescent (right) images are shown. (c) Electron microscopy image of purified CtpS filaments observed in vitro after 10 minutes of incubation in CtpS activity buffer. (d) Localization of E.coli mCherry-EcCtpS in Caulobacter. A Caulobacter strain where Caulobacter CtpS was replaced with EcCtpS at the endogenous ctpS locus and mCherry-EcCtpS was expressed under the xylose promoter (ZG286) were induced with xylose and imaged. Shown are the phase fluorescent merged (left) and fluorescent (right) images. Black scale bars represent 2 μm; white scale bars represent 100 nm.