Figure 2.

Effect of primary UEC CM on the differentiation of DC. (A) Representative FACS histograms of DC differentiated in the presence (Conditioned DC) or absence (Control DC) of primary UEC CM. Primary UEC CM were from epithelial cells isolated from endometrial tissue specimens. Purified epithelial cells were grown to confluence, and CM was harvested from the basolateral compartment following 24 h culture. Unfilled histograms are isotype control antibodies, and the filled histograms indicate specific staining for respective surface antigens. Numbers indicate MFI of a representative experiment (representative of five different experiments). (B) Averaged MFI data for specific antigens expressed by DC from five different donors. Horizontal dotted lines indicate MFI for the expression of surface molecules by Con-DC; values set at 100%. Results are expressed as percent MFI ratio = (MFI of CM-DC÷MFI of Con-DC×100) ± sem; *, P < 0.05.