Figure 8.
Reduced allogeneic, proliferative responses by CM-DC and TGF-β1 DC. Allogeneic PBMC (4×105) were cocultured in triplicate wells with 1 × 105 Con-DC, CM-DC, or TGF-β1 DC (i.e., 4:1 ratio) for 3 days, as described in Materials and Methods. Cells were pulsed with 1 μCi [3H] thymidine and harvested 8 h later onto unifilter 96 GF/C plates for assessment of thymidine incorporation by scintillation counting. Results are reported as mean cpm ± sem for thymidine incorporation. *, P < 0.05, compared with Con-DC.