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Hemolytic activity of ASABF, assayed in the human erythrocyte lysis assay. Red blood cells were incubated for 1 h (37 °C) at increasing concentrations of recombinant ASABF (—▪—). In a parallel series of experiments the recombinant ASABF was adsorbed to anti-ASABF antiserum (PoAb-rASABFSUBDO) (- - • - -). Subsequently, the lysed erythrocytes were determined on the basis of the released hemoglobin. The values represent the means ± SEM of ten experiments each.
