Figure 5.

Expression studies of ASABF gene by application of the in situ hybridization technique. (A,B) Control sponge tissue of non-treated sponge specimens or (C–F) from animals kept for 1 day in the presence of the chelator Dip and subsequently for an additional 1 day in the absence of the chemical, was sliced and the sections were analyzed for the expression of the ASABF gene using the SDASABF DIG-labeled probe. In (A,B,D–F) hybridization was performed with the anti-sense ssDNA probe; (C) had been hybridized with the labeled sense probe. All size bars represent 100 μm.