Table 2.
Expression levels of S. domuncula HAD, ASABF as well as of the caspase genes on the surfaces of control animals, not treated with the Fe++ chelator, or treated with Dip as described under “Experimental Section”. Tissue samples from the Dip-treated animals were taken from the rim of apoptotic region, or after day 5, from the area which previously had been apoptotic and was then cleaned by the gastropod. Then, RNA was extracted and expression levels were quantified through qRT-PCR. Each data point represents the mRNA level of the respective expressed gene (x: HAD, ASABF or caspase) normalized to the amount of β-tubulin transcripts, as means ± SEM (n = five experiments per time point).
| Time point (days) | Gene expression: (mRNAx ± SEM)/mRNAtubulin | |||||
|---|---|---|---|---|---|---|
| HAD | ASABF | Caspase | ||||
| Control (×10−3) | Dip-Treated (×10−3) | Control (×10−2) | Dip-Treated (×10−2) | Control (×10−4) | Dip-Treated (×10−4) | |
| 0 | 2.1 ± 0.3 | 2.2 ± 0.3 | 1.3 ± 0.2 | 1.3 ± 0.2 | 1.3 ± 0.1 | 1.5 ± 0.2 |
| 1 | 2.3 ± 0.3 | 5.3 ± 0.6 | 0.9 ± 0.1 | 2.8 ± 0.3 | 1.6 ± 0.2 | 6.7 ± 0.7 |
| 2 | 2.4 ± 0.4 | 27.1 ± 3.8 | 1.2 ± 0.2 | 4.0 ± 0.5 | 1.9 ± 0.2 | 9.3 ± 1.0 |
| 3 | 1.9 ± 0.2 | 19.9 ± 1.7 | 1.7 ± 0.2 | 6.3 ± 0.8 | 1.8 ± 0.1 | 12.4 ± 1.7 |
| 4 | 1.9 ± 0.2 | 7.2 ± 0.9 | 1.6 ± 0.2 | 9.8 ± 0.9 | 1.4 ± 0.3 | 6.9 ± 0.8 |
| 5 | 2.2 ± 0.5 | 4.2 ± 0.6 | 2.1 ± 0.3 | 6.9 ± 0.7 | 1.8 ± 0.2 | 1.7 ± 0.1 |
| 7 | 2.3 ± 0.3 | 3.4 ± 0.4 | 1.9 ± 0.4 | 3.7 ± 0.5 | 1.8 ± 0.2 | 1.5 ± 0.2 |
| 9 | 2.5 ± 0.4 | 3.1 ± 0.3 | 1.8 ± 0.2 | 4.4 ± 0.5 | 2.1 ± 0.3 | 1.9 ± 0.3 |