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Preferred temperature of the medicinal leech (H. verbana) before and after feeding. The horizontal dashed line shows the acclimation temperature (Ta, 21°C). A, significantly different from the pre-feeding preferred temperature (ANCOVA, planned contrasts, Dunnett's procedure, p < 0.05) (n = 7 for PF, 3, 27 and 51 h, n = 6 for 123 and 243 h). B, significantly different from Ta (one-sample, two-tailed, t-test, p < 0.05 after applying Dunnett's correction). Error bars indicate 1 s.e.m.
