Figure 2.

A, The deduced amino acid sequence of a 35.5-kD cotton annexin cDNA. Potential posttranslational modification sites on the deduced sequence are emphasized as follows: Uppercase, underlined letters, N-myristoylation sites; lowercase, underlined letters, N-glycosylation site; boldface, uppercase letters, cAMP-dependent kinase phosphorylation sites; and boldface, lowercase letters, protein kinase C phosphorylation sites. B, Alignment of annexin repeat domains from cotton (Cot), Arabidopsis (Ara) (Gidrol et al., 1996), and maize (Zea) (Battey et al., 1996). Annexin repeat domains 1 to 4 from Arabidopsis, cotton, and maize are designated Ara1–4, Cot1–4, and Zea1–4, respectively. Underlined are amino acid residues conserved in the type-II Ca²⁺-binding site. The 17-amino acid annexin “endonexin fold” or “consensus sequence” is shown above the alignment, in which h = hydrophobic residue, P = polar residue, and x = variable residue. The 12 domains were aligned by the ClustalW1.7 program, which is available as an Internet service provided by the Human Genome Center at Baylor College of Medicine (Houston, TX).