Figure 5.

Strategy for the construction of domain-deletion mutants of cotton annexin. The four annexin repeat domains are designated inside of the boxes. The numbers below the boxes indicate the first and last numbers of amino acid residues of the cotton annexin expressed by the constructs. The hatched boxes represent the 6xHis tags at the C-terminal ends of recombinant proteins. The eight PCR primers synthesized for mutant construction are designated 1F, 2F, 3F, 4F, 1R, 2R, 3R, and 4R above the arrows that show the sites and orientations of these primers.