Figure 7.

Competition analysis of photoaffinity labeling. The whole recombinant cotton annexin was incubated with [α-³²P]GTP for 10 min under UV irradiation in the presence of unlabeled GTP or ATP at variable concentrations. The protein was extracted and subjected to 10% SDS-PAGE. Top, Autoradiograph of a photolabeled SDS gel. Lanes 1 to 4, Competition of photolabeling by adding unlabeled GTP to a final concentration of 10, 20, 50, and 100 μm, respectively; lanes 5 to 8, competition of photolabeling by adding unlabeled ATP to a final concentration of 10, 20, 50, and 100 μm, respectively. Bottom, Coomassie blue staining of the same gel.