Figure 8.

Mapping of the GTP-binding site on annexin by photolabeling experiment. Recombinant annexin proteins were incubated with [α-³²P]GTP for 10 min under UV irradiation, and the proteins were extracted and subjected to 10% SDS-PAGE. A, SDS-polyacrylamide gel stained with Coomassie blue. Lanes 1 to 8, Purified recombinant annexin proteins of Ann1F1R, Ann1F2R, Ann1F3R, Ann1F4R, Ann2F4R, Ann3F4R, Ann4F4R, and Ann2F3R, respectively. BSA was added to the reactions as the internal control. B, Autoradiograph of the same gel. Note that any annexin mutant missing the C-terminal domain 4 was not labeled.